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src shrna r lentiviral particles  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology src shrna r lentiviral particles
Figure 4. Src <t>shRNA</t> and ERK1/2 shRNA inhibit periodic mechanical stress-induced chondrocyte proliferation and matrix synthesis. A, Chondrocytes were transfected with shRNA targeted to Src or ERK1/2 or with non-targeting (NT) sequences prior to lysis and Western blot for Src and ERK1/2 protein. Either the Src or ERK1/2 shRNA sequence achieved about a 50% reduction in Src or ERK1/2 protein levels. After pretreatment with control shRNA or Src shRNA and ERK1/2 shRNA, rat chondrocytes were cultured for 3 days under static conditions or conditions of periodic mechanical stress 8 h per day prior to proliferation studies. Cell proliferation was analyzed by direct cell counting (B) and CCK-8 assay (C). Rat chondrocytes were cultured in vitro for 8 h with static conditions or periodic mechanical stress. Aggrecan (D) and type II collagen (E) gene expression were measured by quantitative real-time PCR. Cell proliferation and matrix synthesis in the inhibitor pretreatment groups was significantly decreased relative to control in chondrocytes in response to periodic mechanical stress (N = 5, *P < 0.05, Student unpaired t-test).
Src Shrna R Lentiviral Particles, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c-src shrna (r) lentiviral particles  (Santa Cruz Biotechnology)
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Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of c-Src gene silencing results, individual duplex components or plasmids are also available upon request. Suitable
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Figure 4. Src shRNA and ERK1/2 shRNA inhibit periodic mechanical stress-induced chondrocyte proliferation and matrix synthesis. A, Chondrocytes were transfected with shRNA targeted to Src or ERK1/2 or with non-targeting (NT) sequences prior to lysis and Western blot for Src and ERK1/2 protein. Either the Src or ERK1/2 shRNA sequence achieved about a 50% reduction in Src or ERK1/2 protein levels. After pretreatment with control shRNA or Src shRNA and ERK1/2 shRNA, rat chondrocytes were cultured for 3 days under static conditions or conditions of periodic mechanical stress 8 h per day prior to proliferation studies. Cell proliferation was analyzed by direct cell counting (B) and CCK-8 assay (C). Rat chondrocytes were cultured in vitro for 8 h with static conditions or periodic mechanical stress. Aggrecan (D) and type II collagen (E) gene expression were measured by quantitative real-time PCR. Cell proliferation and matrix synthesis in the inhibitor pretreatment groups was significantly decreased relative to control in chondrocytes in response to periodic mechanical stress (N = 5, *P < 0.05, Student unpaired t-test).

Journal: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas

Article Title: Periodic mechanical stress activates MEK1/2-ERK1/2 mitogenic signals in rat chondrocytes through Src and PLCγ1.

doi: 10.1590/s0100-879x2011007500150

Figure Lengend Snippet: Figure 4. Src shRNA and ERK1/2 shRNA inhibit periodic mechanical stress-induced chondrocyte proliferation and matrix synthesis. A, Chondrocytes were transfected with shRNA targeted to Src or ERK1/2 or with non-targeting (NT) sequences prior to lysis and Western blot for Src and ERK1/2 protein. Either the Src or ERK1/2 shRNA sequence achieved about a 50% reduction in Src or ERK1/2 protein levels. After pretreatment with control shRNA or Src shRNA and ERK1/2 shRNA, rat chondrocytes were cultured for 3 days under static conditions or conditions of periodic mechanical stress 8 h per day prior to proliferation studies. Cell proliferation was analyzed by direct cell counting (B) and CCK-8 assay (C). Rat chondrocytes were cultured in vitro for 8 h with static conditions or periodic mechanical stress. Aggrecan (D) and type II collagen (E) gene expression were measured by quantitative real-time PCR. Cell proliferation and matrix synthesis in the inhibitor pretreatment groups was significantly decreased relative to control in chondrocytes in response to periodic mechanical stress (N = 5, *P < 0.05, Student unpaired t-test).

Article Snippet: Src shRNA (r) Lentiviral Particles, ERK1 shRNA (r) Lentiviral Particles, ERK2 shRNA (r) Lentiviral Particles, Control shRNA Lentiviral Particles, and Polybrene were supplied by Santa Cruz.

Techniques: shRNA, Transfection, Lysis, Western Blot, Sequencing, Control, Cell Culture, Cell Counting, CCK-8 Assay, In Vitro, Gene Expression, Real-time Polymerase Chain Reaction

Figure 6. Effect of PP2 and Src shRNA on the expression and phosphorylation of Src, PLCγ1, MEK1/2, and ERK1/2 under con ditions of periodic mechanical stress. After pretreatment with DMSO or Src inhibitor (PP2), or Src shRNA or non-targeting (NT) sequence, rat chondrocytes were cultured in vitro for 1 h under static conditions or conditions of period ic mechanical stress. The expres sion and phosphorylation levels of Src (A), PLCγ1 (B), MEK1/2 (C), and ERK1/2 (D) were detected by Western blotting. For each West ern blot, the corresponding total amount of each signaling protein served as a control. The images above the histogram are repre sentative results of several inde pendent Western blots. The phos phorylation levels of Src-Tyr418 in the PP2 groups were significantly reduced compared to those in the control groups in chondrocytes in response to periodic mechanical stress (N = 5, *P < 0.05, Student unpaired t-test). The phospho rylation levels of PLCγ1-Tyr783, MEK-Ser217/221 and ERK1/2- Thr202/Tyr204 in the PP2 and Src shRNA groups were significantly reduced compared to those in the control groups in chondrocytes in response to periodic mechanical stress (N = 5, *P < 0.05, Student unpaired t-test).

Journal: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas

Article Title: Periodic mechanical stress activates MEK1/2-ERK1/2 mitogenic signals in rat chondrocytes through Src and PLCγ1.

doi: 10.1590/s0100-879x2011007500150

Figure Lengend Snippet: Figure 6. Effect of PP2 and Src shRNA on the expression and phosphorylation of Src, PLCγ1, MEK1/2, and ERK1/2 under con ditions of periodic mechanical stress. After pretreatment with DMSO or Src inhibitor (PP2), or Src shRNA or non-targeting (NT) sequence, rat chondrocytes were cultured in vitro for 1 h under static conditions or conditions of period ic mechanical stress. The expres sion and phosphorylation levels of Src (A), PLCγ1 (B), MEK1/2 (C), and ERK1/2 (D) were detected by Western blotting. For each West ern blot, the corresponding total amount of each signaling protein served as a control. The images above the histogram are repre sentative results of several inde pendent Western blots. The phos phorylation levels of Src-Tyr418 in the PP2 groups were significantly reduced compared to those in the control groups in chondrocytes in response to periodic mechanical stress (N = 5, *P < 0.05, Student unpaired t-test). The phospho rylation levels of PLCγ1-Tyr783, MEK-Ser217/221 and ERK1/2- Thr202/Tyr204 in the PP2 and Src shRNA groups were significantly reduced compared to those in the control groups in chondrocytes in response to periodic mechanical stress (N = 5, *P < 0.05, Student unpaired t-test).

Article Snippet: Src shRNA (r) Lentiviral Particles, ERK1 shRNA (r) Lentiviral Particles, ERK2 shRNA (r) Lentiviral Particles, Control shRNA Lentiviral Particles, and Polybrene were supplied by Santa Cruz.

Techniques: shRNA, Expressing, Phospho-proteomics, Sequencing, Cell Culture, In Vitro, Western Blot, Control